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spin column based method  (Qiagen)


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    Structured Review

    Qiagen spin column based method
    Spin Column Based Method, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 4520 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/spin+column+method/QIAamp+Circulating+Nucleic+Acid+Kit/pmc12652532-240-8-17
    Average 99 stars, based on 4520 article reviews
    spin column based method - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Devices and methods for plasmid purification
    Article Snippet: .. Additionally, plasmid DNA was purified in duplicate using a spin column method (Qiagen Cat. #27104) according to the manufacturer's protocol. .. The plasmid DNAs were tested using a ToxinSensor Chromogenic LAL Endotoxin Assay Kit from Genscript (Cat. L00350C).

    Purification:

    Article Title: Devices and methods for plasmid purification
    Article Snippet: .. Additionally, plasmid DNA was purified in duplicate using a spin column method (Qiagen Cat. #27104) according to the manufacturer's protocol. .. The plasmid DNAs were tested using a ToxinSensor Chromogenic LAL Endotoxin Assay Kit from Genscript (Cat. L00350C).

    DNA Extraction:

    Article Title: A Comparative Spatial and Climate Analysis of Human Granulocytic Anaplasmosis and Human Babesiosis in New York State (2013-2018).
    Article Snippet: I. scapularis were identified under a dissecting microscope (Model SMZ1000, Nikon, Tokyo, Japan) to species utilizing dichotomous keys (Keirans and Clifford 1978, Keirans et al. 1996) and stored in 99.5% ethanol at −20°C until DNA extraction. .. I. scapularis samples collected between 2007 and 2012 underwent total genomic DNA extraction via spin column method (DNeasy Blood and Tissue Kit, Qiagen USA, Germantown, MD), and were tested for the presence of (target gene): A. phagocytophilum (msp3), B. microti (16S-like rRNA), and B. burgdorferi (ospA) using a triplex Polymerase Chain Reaction (PCR) assay as described elsewhere (Prusinski et al. 2014). .. I. scapularis samples collected between 2013 and 2018 underwent automated total genomic DNA extraction via Qiagen QIAcube HT using QIAamp 96 kit (Qiagen USA, Germantown, MD) according to manufacturer protocols.

    Article Title: Sea ice loss increases genetic isolation in a high Arctic ungulate metapopulation.
    Article Snippet: .. DNA extraction and microsatellite genotyping DNA was extracted using the Chelex 100 method for hair samples (Walsh, Metzger, & Higuchi, 1991) and the spin column method (QIAGEN) for all other sample types (see Supplementary Methods S1 for detailed procedures). ..

    Polymerase Chain Reaction:

    Article Title: A Comparative Spatial and Climate Analysis of Human Granulocytic Anaplasmosis and Human Babesiosis in New York State (2013-2018).
    Article Snippet: I. scapularis were identified under a dissecting microscope (Model SMZ1000, Nikon, Tokyo, Japan) to species utilizing dichotomous keys (Keirans and Clifford 1978, Keirans et al. 1996) and stored in 99.5% ethanol at −20°C until DNA extraction. .. I. scapularis samples collected between 2007 and 2012 underwent total genomic DNA extraction via spin column method (DNeasy Blood and Tissue Kit, Qiagen USA, Germantown, MD), and were tested for the presence of (target gene): A. phagocytophilum (msp3), B. microti (16S-like rRNA), and B. burgdorferi (ospA) using a triplex Polymerase Chain Reaction (PCR) assay as described elsewhere (Prusinski et al. 2014). .. I. scapularis samples collected between 2013 and 2018 underwent automated total genomic DNA extraction via Qiagen QIAcube HT using QIAamp 96 kit (Qiagen USA, Germantown, MD) according to manufacturer protocols.

    Isolation:

    Article Title: MTHFR C677T and MTR A2756G Gene Polymorphism in Neural Tube Defect Patients and Its Association with Red Blood Cell Folate Level in Eastern Indian Population
    Article Snippet: .. Genomic DNA was isolated from 200 μl peripheral blood collected in the EDTA microtainer using Qiagen Spin column method (Qiagen, Germany) according to the manufacturer's instructions. .. Quantification of extracted DNA was done using NanoDrop spectrophotometer.

    Article Title: Effects of Sudarshan KriyaYoga and Advanced Meditation Program on Genetic Expression of Pro-inflammatory and Antioxidants Genes
    Article Snippet: .. PBMCs were separated by Ficoll-Paque density gradient centrifugation (Ficoll®-Paque Premium GE17-5442-02; Cytiva, Marlborough, MA, USA) and used for RNA isolation using a commercial spin column method (Qiagen, Inc., Limburg, Netherlands). .. The obtained RNA was quantified by spectrophotometry (MultiskanTM GO Microplate Spectrophotometer; Thermo Fisher Scientific Inc., Waltham, MA, USA).

    Comparison:

    Article Title: Genome-wide analysis of aberrant position and sequence of plasma DNA fragment ends in patients with cancer.
    Article Snippet: Karan K. Budhraja1†, Bradon R. McDonald1†, Michelle D. Stephens1†, Tania Contente-Cuomo2, Havell Markus3, Maria Farooq4, Patricia F. Favaro1, Sydney Connor5, Sara A. Byron2, Jan B. Egan6, Brenda Ernst6, Timothy K. McDaniel2‡, Aleksandar Sekulic6, Nhan L. Tran6, Michael D. Prados7, Mitesh J. Borad6, Michael E. Berens2, Barbara A. Pockaj6, Patricia M. LoRusso8, Alan Bryce6, Jeffrey M. Trent2, Muhammed Murtaza1*

    Clinical Proteomics:

    Article Title: Genome-wide analysis of aberrant position and sequence of plasma DNA fragment ends in patients with cancer.
    Article Snippet: Karan K. Budhraja1†, Bradon R. McDonald1†, Michelle D. Stephens1†, Tania Contente-Cuomo2, Havell Markus3, Maria Farooq4, Patricia F. Favaro1, Sydney Connor5, Sara A. Byron2, Jan B. Egan6, Brenda Ernst6, Timothy K. McDaniel2‡, Aleksandar Sekulic6, Nhan L. Tran6, Michael D. Prados7, Mitesh J. Borad6, Michael E. Berens2, Barbara A. Pockaj6, Patricia M. LoRusso8, Alan Bryce6, Jeffrey M. Trent2, Muhammed Murtaza1*

    Magnetic Beads:

    Article Title: Genome-wide analysis of aberrant position and sequence of plasma DNA fragment ends in patients with cancer.
    Article Snippet: Karan K. Budhraja1†, Bradon R. McDonald1†, Michelle D. Stephens1†, Tania Contente-Cuomo2, Havell Markus3, Maria Farooq4, Patricia F. Favaro1, Sydney Connor5, Sara A. Byron2, Jan B. Egan6, Brenda Ernst6, Timothy K. McDaniel2‡, Aleksandar Sekulic6, Nhan L. Tran6, Michael D. Prados7, Mitesh J. Borad6, Michael E. Berens2, Barbara A. Pockaj6, Patricia M. LoRusso8, Alan Bryce6, Jeffrey M. Trent2, Muhammed Murtaza1*

    Gradient Centrifugation:

    Article Title: Effects of Sudarshan KriyaYoga and Advanced Meditation Program on Genetic Expression of Pro-inflammatory and Antioxidants Genes
    Article Snippet: .. PBMCs were separated by Ficoll-Paque density gradient centrifugation (Ficoll®-Paque Premium GE17-5442-02; Cytiva, Marlborough, MA, USA) and used for RNA isolation using a commercial spin column method (Qiagen, Inc., Limburg, Netherlands). .. The obtained RNA was quantified by spectrophotometry (MultiskanTM GO Microplate Spectrophotometer; Thermo Fisher Scientific Inc., Waltham, MA, USA).



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